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NEFH antibody (Neurofilament, Heavy Polypeptide) (CF®555) Primary Antibody

NEFH Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, IHC (f) Host: Mouse Monoclonal RT-97 CF®555
Catalog No. ABIN6172788
$270.77
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  • Target See all NEFH Antibodies
    NEFH
    Reactivity
    • 115
    • 97
    • 78
    • 68
    • 49
    • 32
    • 13
    • 12
    • 11
    • 8
    • 8
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Pig
    Host
    • 86
    • 50
    • 12
    Mouse
    Clonality
    • 96
    • 52
    Monoclonal
    Conjugate
    • 98
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEFH antibody is conjugated to CF®555
    Application
    • 92
    • 49
    • 46
    • 33
    • 31
    • 28
    • 27
    • 19
    • 14
    • 14
    • 14
    • 10
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Neurofilament (NF-H) (RT-97), CF555 Conjugate
    Characteristics
    This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Triton-X 100 insoluble protein fraction of rat brain
    Clone
    RT-97
    Isotype
    IgG1
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Brain, Neuroblastoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    NEFH
    Alternative Name
    Neurofilament (NF-H) (NEFH Products)
    Synonyms
    NF-H, NF200, Nfh, mKIAA0845, NFH, NEFH, neurofilament heavy polypeptide, neurofilament, heavy polypeptide, neurofilament heavy, MCYG_06231, Nefh, NEFH
    Background
    NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200  kDa, Neurofilament Triplet H Protein, NF-H, NF200
    Molecular Weight
    200 kDa
    Gene ID
    4744, 198760
    UniProt
    P12036
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