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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (CF®555) Primary Antibody

CDKN1B Reactivity: Human, Mouse, Rat, Monkey FACS, IF, IHC (f), WB Host: Mouse Monoclonal DCS-72-F6 CF®555
Catalog No. ABIN6174346
$270.77
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  • Target See all CDKN1B Antibodies
    CDKN1B
    Reactivity
    • 313
    • 144
    • 125
    • 41
    • 11
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Monkey
    Host
    • 249
    • 65
    • 14
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 261
    • 71
    Monoclonal
    Conjugate
    • 137
    • 26
    • 23
    • 19
    • 18
    • 16
    • 16
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This CDKN1B antibody is conjugated to CF®555
    Application
    • 223
    • 164
    • 88
    • 75
    • 55
    • 50
    • 32
    • 29
    • 20
    • 16
    • 12
    • 8
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Purpose
    Mouse Monoclonal anti-p27 / KIP1 (DCS-72.F6), CF555 Conjugate
    Characteristics
    This antibody recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Mouse recombinant p27 protein
    Clone
    DCS-72-F6
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    CDKN1B
    Alternative Name
    p27 / KIP1 (CDKN1B Products)
    Synonyms
    CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B, Kip1, p27, FAM14D, ISG12, ISG12A, P27, Cdki1b, AA408329, AI843786, p27Kip1, cdkn1bl, cyclin dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1Bb, interferon alpha inducible protein 27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1Ba, CDKN1B, cdkn1bb, IFI27, Cdkn1b, cdkn1ba
    Background
    CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4
    Molecular Weight
    25-26 kDa
    Gene ID
    1027, 238990
    UniProt
    P46527
    Pathways
    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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