CD11b antibody (CF®647)

Catalog No. ABIN6176055

Product Details anti-CD11b Antibody (CF®647)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rabbit, Baboon, Rhesus Monkey, Chimpanzee

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Rat Monoclonal anti-CD11b (M1/70), CF647 Conjugate

Characteristics: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin i±M combines with the Integrin i²2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin i±M/i²2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: B10 mouse spleen cells enriched for T lymphocytes

Clone: M1-70

Isotype: IgG2b kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mLFlow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

Monocytes & granulocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b (ITGAM Products)

Synonyms: CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody

Background: CD11 antigen-like family member B|CD11b/CD18|CD49d|Cell surface glycoprotein MAC-1 subunit alpha|Complement Component Receptor 3 Alpha|CR3 Alpha Chain (CR3A)|Integrin alpha-M|Integrin beta 2 alpha subunit|ITGAM|Leukocyte adhesion receptor MO1, Ly-40|Mac-1a|MAC1|Mac1, alpha subunit|MAC1A|Macrophage antigen alpha polypeptide, MO1A|Neutrophil adherence receptor alpha M subunit

Molecular Weight: 95 kDa & 170 kDa

Gene ID: 3684, 172631

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation