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CD45RA antibody

CD45RA Reactivity: Human FACS, IF, IHC (f) Host: Mouse Monoclonal 158-4D3 unconjugated
Catalog No. ABIN6177185
  • Target See all CD45RA Antibodies
    CD45RA
    Reactivity
    • 98
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 109
    • 1
    Mouse
    Clonality
    • 109
    • 1
    Monoclonal
    Conjugate
    • 27
    • 15
    • 14
    • 8
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD45RA antibody is un-conjugated
    Application
    • 108
    • 29
    • 29
    • 26
    • 18
    • 16
    • 15
    • 15
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-CD45RA (158-4D3), Purified, BSA-free
    Characteristics
    This antibody recognizes a protein of 205 kDa-220 kDa, identified as CD45RA [Workshop V, Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4 T-cells, 50 % of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Purification
    Purified
    Immunogen
    Stimulated human leukocytes
    Clone
    158-4D3
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CD45RA Primary Antibody
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Daudi, Raji, IM9 and human lymphocytes. Human lymph nodes and tonsils.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS (no BSA, no azide)
    Preservative
    Azide free
  • Target
    CD45RA
    Alternative Name
    CD45RA (CD45RA Products)
    Synonyms
    B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Lca antibody, RT7 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody
    Background
    B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
    Molecular Weight
    205-220 kDa
    Gene ID
    5788, 654514
    UniProt
    P08575
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