Recombinant Keratin Acidic (AE1) antibody (CF®405S)

Catalog No. ABIN6178744

Product Details

Target: Keratin Acidic (AE1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: CF®405S

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Recombinant Rabbit Monoclonal anti-Cytokeratin, Acidic (KRTL/1577R), CF405S Conjugate

Characteristics: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human KRT77 protein fragment

Clone: KRTL-1577R

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Skin, Squamous cell carcinoma (SCC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Cytokeratin, Acidic (AE1 Products)

Background: K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B

Molecular Weight: 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19)

Gene ID: 374454, 334989

UniProt: Q7Z794