KRT18 antibody (Keratin 18) (PerCP)

Details for Product anti-KRT18 Antibody No. ABIN6179423
Antigen
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human
882
225
215
126
95
64
64
52
48
37
35
33
33
33
24
14
11
9
6
3
2
2
2
1
1
Host
Mouse
592
327
14
7
5
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This KRT18 antibody is conjugated to PerCP
55
41
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
7
3
3
3
3
3
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
563
398
366
365
241
193
169
86
77
76
41
40
30
17
14
11
2
1
1
1
Options
Purpose Mouse Monoclonal anti-Cytokeratin 18 (KRT18/835), Per-CP Conjugate
Immunogen Recombinant full-length human KRT18 protein
Clone KRT18-835
Isotype IgG1
Characteristics This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Plasmids, Primers & others Plasmids, Primers & others KRT18 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18
Molecular Weight 45 kDa
Gene ID 3875, 406013
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Does not react with mouse, rat, sheep, hamster, cow, dog or pig, others not known
  • Optimal dilution for a specific application should be determined by user
Comment

MCF-7, HeLa, A431 cells or Breast Cancer

Restrictions For Research Use only
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Image no. 1 for anti-Keratin 18 (KRT18) antibody (PerCP) (ABIN6179423) anti-Keratin 18 (KRT18) antibody (PerCP) (Image 1)
Image no. 2 for anti-Keratin 18 (KRT18) antibody (PerCP) (ABIN6179423) anti-Keratin 18 (KRT18) antibody (PerCP) (Image 2)
Image no. 3 for anti-Keratin 18 (KRT18) antibody (PerCP) (ABIN6179423) anti-Keratin 18 (KRT18) antibody (PerCP) (Image 3)
Image no. 4 for anti-Keratin 18 (KRT18) antibody (PerCP) (ABIN6179423) anti-Keratin 18 (KRT18) antibody (PerCP) (Image 4)
Image no. 5 for anti-Keratin 18 (KRT18) antibody (PerCP) (ABIN6179423) anti-Keratin 18 (KRT18) antibody (PerCP) (Image 5)