MAML2 antibody (CF®594)

Catalog No. ABIN6187052

Product Details anti-MAML2 Antibody (CF®594)

Target: MAML2 (Mastermind-Like 2 (MAML2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML2 antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt)

Purpose: Mouse Monoclonal anti-MAML2 (MAML2/1302), CF594 Conjugate

Characteristics: MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J?, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length MAML2

Clone: MAML2-1302

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for MAML2

Target: MAML2 (Mastermind-Like 2 (MAML2))

Alternative Name: MAML2 (MAML2 Products)

Synonyms: 5930431H10 antibody, BC032967 antibody, MAM-3 antibody, MAM2 antibody, MAM3 antibody, MLL-MAML2 antibody, mastermind like 2 (Drosophila) antibody, mastermind-like transcriptional coactivator 2 antibody, mastermind like transcriptional coactivator 2 antibody, Maml2 antibody, MAML2 antibody

Background: Mam-2, MAML2, Mastermind-like protein 2

Molecular Weight: 125 kDa

Gene ID: 84441