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Nuclear Antigen antibody (CF®568)

Reactivity: Human, Non-Human Primate IF, ICC, FACS, IHC (fro), IP Host: Mouse Monoclonal 235-1 CF®568
Catalog No. ABIN6190804
  • Target
    Nuclear Antigen
    Reactivity
    • 32
    • 15
    • 14
    • 10
    • 3
    Human, Non-Human Primate
    Host
    • 29
    • 3
    Mouse
    Clonality
    • 32
    Monoclonal
    Conjugate
    • 16
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    CF®568
    Application
    • 28
    • 27
    • 24
    • 14
    • 13
    • 10
    • 7
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Purpose
    Mouse Monoclonal anti-Human Nuclear Antigen (235-1), CF568 Conjugate
    Characteristics
    This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Nuclei of human myeloid leukemia biopsy cells
    Clone
    235-1
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology Frozen 0.5-1.0 μg/mL
    • Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunoprecipitation 0.5-1 μg/500 μg protein lysate
    • Does not react with mouse, rat or chicken, others not known
    • Optimal dilution for a specific application should be determined by user
    Comment

    All human cells. Tonsil

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Nuclear Antigen
    Background
    HNA, HuNu
    Molecular Weight
    70 kDa & 80 kDa
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