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CD3 epsilon antibody (Cytoplasmic Domain)
CD3E
Reactivity: Human, Mouse, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig
FACS, WB, IF, IHC (p)
Host: Mouse
Monoclonal
PC3-188A
unconjugated
Product Details anti-CD3 epsilon Antibody
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Target
See all CD3 epsilon (CD3E) Antibodies
CD3 epsilon (CD3E)
Binding Specificity
All epitopes for CD3 epsilon antibodies
AA 156-168, Cytoplasmic Domain
Reactivity
All reactivities for CD3 epsilon antibodies
Human, Mouse, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig
Host
All hosts for CD3 epsilon antibodies
Mouse
Clonality
All clonalities for CD3 epsilon antibodies
Monoclonal
Conjugate
All conjugates for CD3 epsilon antibodies
This CD3 epsilon antibody is un-conjugated
Application
All applications for CD3 epsilon antibodies
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Protein G affinity chromatography
Immunogen
Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Clone
PC3-188A
Isotype
IgG1
Top Product
Discover our top product CD3E Primary Antibody
Alternatives
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anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibody
CD3E
Reactivity: Human, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig, Chicken
FACS, WB, IF
Host: Mouse
Monoclonal
PC3-188A
unconjugated
Application Details
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Application Notes
WB: 1-2 μg/mL IHC (FFPE): 1-2 μg/mL for 30 min at RT Flow Cytometry: 0.5-1 μg/million cells in 0.1ml Immunofluorescence: 1-2 μg/mL Optimal dilution of the CD3e antibody should be determined by the researcher.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Target Details for CD3 epsilon
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Target
CD3 epsilon (CD3E)
Alternative Name
CD3e (CD3E Products )
Synonyms
cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
Background
Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Gene ID
916
UniProt
P07766
Pathways
TCR Signaling , CXCR4-mediated Signaling Events , Ubiquitin Proteasome Pathway
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