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CD3 epsilon antibody (Cytoplasmic Domain)

CD3E Reactivity: Human, Mouse, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig FACS, WB, IF, IHC (p) Host: Mouse Monoclonal PC3-188A unconjugated
Catalog No. ABIN6252415
  • Target See all CD3 epsilon (CD3E) Antibodies
    CD3 epsilon (CD3E)
    Binding Specificity
    • 24
    • 20
    • 16
    • 16
    • 16
    • 15
    • 12
    • 9
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 156-168, Cytoplasmic Domain
    Reactivity
    • 217
    • 161
    • 43
    • 41
    • 18
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    Human, Mouse, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig
    Host
    • 143
    • 117
    • 33
    • 22
    • 20
    • 8
    • 1
    Mouse
    Clonality
    • 217
    • 121
    • 6
    Monoclonal
    Conjugate
    • 126
    • 31
    • 27
    • 20
    • 15
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD3 epsilon antibody is un-conjugated
    Application
    • 234
    • 157
    • 86
    • 81
    • 71
    • 70
    • 27
    • 27
    • 24
    • 22
    • 14
    • 14
    • 13
    • 13
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogen
    Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
    Clone
    PC3-188A
    Isotype
    IgG1
    Top Product
    Discover our top product CD3E Primary Antibody
  • Application Notes
    WB: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 min at RT

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Optimal dilution of the CD3e antibody should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    CD3 epsilon (CD3E)
    Alternative Name
    CD3e (CD3E Products)
    Synonyms
    cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
    Background
    Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
    Gene ID
    916
    UniProt
    P07766
    Pathways
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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