GFP antibody

Catalog No. ABIN1440019

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea aequorea

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.

Characteristics: Green Fluorescent Protein

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Concentration: 3 mg/mL

Buffer: Polyclonal antibody supplied as a 200 µL (3 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-GFP antibody (ABIN1440019)

Tiede, Bedford, Heseltine, Smith, Wijetunga, Ross, AlQallaf, Roberts, Balls, Curd, Hughes, Martin, Needham, Zanetti-Domingues, Sadigh, Peacock, Tang, Gibson, Kyle, Platt, Ingram, Taylor, Coletta et al.: "Affimer proteins are versatile and renewable affinity reagents. ..." in: eLife, Vol. 6, (2018) (PubMed).

Gabriel, Belge, Gassama, Debaix, Luciani, Fehr, Devuyst: "Bone marrow transplantation improves proximal tubule dysfunction in a mouse model of Dent disease." in: Kidney international, Vol. 91, Issue 4, pp. 842-855, (2017) (PubMed).

Roux, Wu, McIntosh, Glowatzki: "Assessment of the expression and role of the α1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea." in: Journal of neurophysiology, Vol. 116, Issue 2, pp. 479-92, (2017) (PubMed).

Krishnamurthy, Khamo, Mei, Turgeon, Ashraf, Mondal, Patel, Risner, Cho, Yang, Zhang: "Reversible optogenetic control of kinase activity during differentiation and embryonic development." in: Development (Cambridge, England), Vol. 143, Issue 21, pp. 4085-4094, (2017) (PubMed).

Bugalhão, Mota, Franco: "Identification of regions within the Legionella pneumophila VipA effector protein involved in actin binding and polymerization and in interference with eukaryotic organelle trafficking." in: MicrobiologyOpen, Vol. 5, Issue 1, pp. 118-33, (2016) (PubMed).

Sargiannidou, Kim, Kyriakoudi, Eun, Kleopa: "A start codon CMT1X mutation associated with transient encephalomyelitis causes complete loss of Cx32." in: Neurogenetics, Vol. 16, Issue 3, pp. 193-200, (2016) (PubMed).

Issa, Haeffele, Agarwal, Bergles, Young, Yue: "Multiscale optical Ca2+ imaging of tonal organization in mouse auditory cortex." in: Neuron, Vol. 83, Issue 4, pp. 944-59, (2014) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Target Type: Viral Protein

Background: Synonyms: Green fluorescent protein antibody.