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KIT antibody (pTyr703)

KIT Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6255994
  • Target See all KIT Antibodies
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Binding Specificity
    • 55
    • 55
    • 35
    • 28
    • 23
    • 23
    • 23
    • 21
    • 16
    • 16
    • 16
    • 16
    • 16
    • 16
    • 16
    • 14
    • 13
    • 12
    • 12
    • 12
    • 10
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    pTyr703
    Reactivity
    • 668
    • 226
    • 65
    • 29
    • 28
    • 28
    • 17
    • 13
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 601
    • 101
    • 34
    • 2
    • 1
    Rabbit
    Clonality
    • 593
    • 146
    • 1
    Polyclonal
    Conjugate
    • 226
    • 80
    • 77
    • 75
    • 60
    • 60
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 11
    • 10
    • 10
    • 10
    • 10
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KIT antibody is un-conjugated
    Application
    • 429
    • 390
    • 287
    • 147
    • 91
    • 91
    • 64
    • 47
    • 46
    • 46
    • 30
    • 24
    • 10
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Phospho-c-Kit (Tyr703) Antibody detects endogenous levels of c-Kit only when phosphorylated at Tyrosine 703.
    Predicted Reactivity
    Pig,Bovine,Horse,Rabbit,Dog,Chicken
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human c-Kit around the phosphorylation site of Tyr703.
    Isotype
    IgG
    Top Product
    Discover our top product KIT Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Alternative Name
    KIT (KIT Products)
    Synonyms
    C-Kit antibody, CD117 antibody, PBT antibody, SCFR antibody, Bs antibody, Fdc antibody, Gsfsco1 antibody, Gsfsco5 antibody, Gsfsow3 antibody, SCO1 antibody, SCO5 antibody, SOW3 antibody, Ssm antibody, Tr-kit antibody, W antibody, c-KIT antibody, c-kit antibody, MGF antibody, KIT antibody, C-KIT antibody, kit antibody, receptor antibody, sparse antibody, Xkl-1 antibody, kit-a antibody, kit-b antibody, kl1-A antibody, krk1 antibody, pbt antibody, scfr antibody, KIT proto-oncogene receptor tyrosine kinase antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antibody, KIT proto-oncogene receptor tyrosine kinase S homeolog antibody, KIT antibody, Kit antibody, kita antibody, kit antibody, kit.S antibody
    Background

    Description: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

    Gene: KIT

    Molecular Weight
    145kDa
    Gene ID
    3815
    UniProt
    P10721
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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