EIF5B antibody (C-Term)

Catalog No. ABIN6257600

Product Details anti-EIF5B Antibody

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: EIF5B Antibody detects endogenous levels of total EIF5B.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF5B, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Alternative Name: EIF5B (EIF5B Products)

Synonyms: CIF2 antibody, EIF5B antibody, MGC134193 antibody, im:6912504 antibody, si:ch211-254d18.3 antibody, AO090003000953 antibody, DDBDRAFT_0206214 antibody, DDBDRAFT_0234259 antibody, DDB_0206214 antibody, DDB_0234259 antibody, IF2 antibody, A030003E17Rik antibody, BC018347 antibody, eukaryotic translation initiation factor 5B antibody, translation initiation factor IF-2 antibody, EIF5B antibody, eif5b antibody, EDI_190850 antibody, AOR_1_1690154 antibody, CMU_007690 antibody, POSPLDRAFT_134975 antibody, POSPLDRAFT_134953 antibody, eIF5b antibody, MCYG_03474 antibody, PITG_20386 antibody, eif5B antibody, MGYG_03082 antibody, PGTG_13938 antibody, Eif5b antibody

Background:

Description: Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.

Gene: EIF5B

Molecular Weight: 139 kDa

Gene ID: 9669

UniProt: O60841