CHAF1A antibody (Internal Region)

Catalog No. ABIN6257706

The Rabbit Polyclonal anti-CHAF1A antibody has been validated for WB and ELISA. It is suitable to detect CHAF1A in samples from Human, Mouse and Rat.

Quick Overview for CHAF1A antibody (Internal Region) (ABIN6257706)

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHAF1A Antibody

Binding Specificity: Internal Region

Specificity: CAF1A Antibody detects endogenous levels of total CAF1A.

Predicted Reactivity: Bovine,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CAF1A, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CHAF1A

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Alternative Name: CHAF1A

Background:

Description: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).

Gene: CHAF1A

Molecular Weight: 150 kDa

Gene ID: 10036

UniProt: Q13111