PTPN22 antibody (C-Term)

Catalog No. ABIN6258258

Product Details anti-PTPN22 Antibody

Target: PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTPN22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PTPN22 Antibody detects endogenous levels of total PTPN22.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PTPN22, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PTPN22

Target: PTPN22 (Tyrosine-Protein Phosphatase Non-Receptor Type 22 (PTPN22))

Alternative Name: PTPN22 (PTPN22 Products)

Synonyms: 70zpep antibody, PEP antibody, Ptpn8 antibody, LYP antibody, LYP1 antibody, LYP2 antibody, PTPN8 antibody, protein tyrosine phosphatase, non-receptor type 22 (lymphoid) antibody, protein tyrosine phosphatase, non-receptor type 22 antibody, Ptpn22 antibody, PTPN22 antibody

Background:

Description: Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed:16461343, PubMed:18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed:21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed:16461343). Dephosphorylates the immune system activator SKAP2 (PubMed:21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed:23871208). Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed:23991106).

Gene: PTPN22

Molecular Weight: 91 kDa

Gene ID: 26191

UniProt: Q9Y2R2