POU4F1 antibody (Internal Region)

Catalog No. ABIN6258459

Product Details anti-POU4F1 Antibody

Target: POU4F1 (POU Domain, Class 4, Transcription Factor 1 (POU4F1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: POU4F1 Antibody detects endogenous levels of total POU4F1.

Predicted Reactivity: Pig,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POU4F1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POU4F1

Target: POU4F1 (POU Domain, Class 4, Transcription Factor 1 (POU4F1))

Alternative Name: POU4F1 (POU4F1 Products)

Synonyms: BRN3A antibody, Oct-T1 antibody, RDC-1 antibody, brn-3A antibody, POU4F1 antibody, Brn-3a antibody, Brn-3 antibody, Brn-3.0 antibody, Brn3 antibody, Brn3.0 antibody, Brn3a antibody, E130119J07Rik antibody, brn3.0 antibody, brn3a antibody, brn3a-A antibody, oct-t1 antibody, rdc-1 antibody, POU class 4 homeobox 1 antibody, POU domain, class 4, transcription factor 1 antibody, POU class 4 homeobox 1 L homeolog antibody, POU4F1 antibody, Pou4f1 antibody, pou4f1 antibody, Tsp_01181 antibody, pou4f1.L antibody

Background:

Description: Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.

Gene: POU4F1

Molecular Weight: 42 kDa

Gene ID: 5457

UniProt: Q01851

Pathways: Feeding Behaviour