ATP5A1 antibody (Internal Region)

Catalog No. ABIN6258538

Product Details anti-ATP5A1 Antibody

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ATP5A1 Antibody detects endogenous levels of total ATP5A1.

Predicted Reactivity: Pig,Bovine,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP5A1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5A1

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Alternative Name: ATP5A1 (ATP5A1 Products)

Synonyms: atp5a antibody, MGC108350 antibody, AI035633 antibody, AL022851 antibody, AL023067 antibody, Atpm antibody, D18Ertd206e antibody, Mom2 antibody, ATP5A antibody, ATP5AL2 antibody, ATPM antibody, MC5DN4 antibody, MOM2 antibody, OMR antibody, ORM antibody, hATP1 antibody, zgc:154103 antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, ATP synthase subunit alpha, mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 antibody, atp5a1.L antibody, ATP5A1 antibody, atp5a1 antibody, LOC100395383 antibody, Atp5a1 antibody

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).

Gene: ATP5A1

Molecular Weight: 60 kDa

Gene ID: 498

UniProt: P25705

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process