USP44 antibody (Internal Region)

Catalog No. ABIN6258564

Product Details anti-USP44 Antibody

Target: USP44 (Ubiquitin Specific Peptidase 44 (USP44))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP44 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: USP44 Antibody detects endogenous levels of total USP44.

Predicted Reactivity: Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human USP44, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for USP44

Target: USP44 (Ubiquitin Specific Peptidase 44 (USP44))

Alternative Name: USP44 (USP44 Products)

Synonyms: usp44 antibody, USP49 antibody, E430004F17Rik antibody, zgc:55661 antibody, ubiquitin specific peptidase 44 antibody, ubiquitin specific peptidase 44 L homeolog antibody, ubiquitin specific peptidase 44 S homeolog antibody, USP44 antibody, usp44.L antibody, usp44.S antibody, usp44 antibody, Usp44 antibody

Background:

Description: Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.

Gene: USP44

Molecular Weight: 81 kDa

Gene ID: 84101

UniProt: Q9H0E7

Pathways: M Phase