BAZ2A antibody (N-Term)

Catalog No. ABIN6258712

Product Details anti-BAZ2A Antibody

Target: BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A (BAZ2A))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAZ2A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: BAZ2A Antibody detects endogenous levels of total BAZ2A.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BAZ2A, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BAZ2A

Target: BAZ2A (Bromodomain Adjacent To Zinc Finger Domain, 2A (BAZ2A))

Alternative Name: BAZ2A (BAZ2A Products)

Synonyms: zgc:101601 antibody, tip5 antibody, bax2a antibody, walp3 antibody, TIP5 antibody, WALp3 antibody, AA415431 antibody, C030005G16Rik antibody, C78388 antibody, Tip5 antibody, Walp3 antibody, mKIAA0314 antibody, bromodomain adjacent to zinc finger domain, 2A antibody, bromodomain adjacent to zinc finger domain 2A antibody, bromodomain adjacent to zinc finger domain 2A L homeolog antibody, Baz2a antibody, baz2a antibody, BAZ2A antibody, baz2a.L antibody

Background:

Description: Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter, pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).

Gene: BAZ2A

Molecular Weight: 210 kDa

Gene ID: 11176

UniProt: Q9UIF9