GPR31 antibody (Internal Region)

Catalog No. ABIN6258802

Product Details anti-GPR31 Antibody

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: GPR31 Antibody detects endogenous levels of total GPR31.

Predicted Reactivity: Pig,Bovine,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GPR31, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GPR31

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31 (GPR31 Products)

Synonyms: 12-HETER antibody, HETER antibody, HETER1 antibody, GPR31 antibody, GPR31c(t) antibody, Gpr31 antibody, Gpr31c antibody, G protein-coupled receptor 31 antibody, G protein-coupled receptor 31, D17Leh66b region antibody, GPR31 antibody, Gpr31 antibody, Gpr31b antibody

Background:

Description: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.

Gene: GPR31

Molecular Weight: 33 kDa

Gene ID: 2853

UniProt: O00270