HMGB2 antibody (Internal Region)

Catalog No. ABIN6258843

Product Details anti-HMGB2 Antibody

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: HMGB2 Antibody detects endogenous levels of total HMGB2.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HMGB2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HMGB2

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: HMGB2 (HMGB2 Products)

Synonyms: HMG2 antibody, C80539 antibody, HMG-2 antibody, Hmg2 antibody, HIGH MOBILITY GROUP BETA 1 antibody, HMG BETA 1 antibody, NFD02 antibody, NFD2 antibody, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR GROUP D 02 antibody, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR GROUP D 2 antibody, high mobility group B2 antibody, hmgb2 antibody, hmgb2l antibody, im:6909096 antibody, wu:fa20b02 antibody, zgc:101854 antibody, wu:fb22b10 antibody, wu:fc95d12 antibody, zgc:123215 antibody, high mobility group box 2 antibody, high mobility group box 2 S homeolog antibody, high mobility group B2 antibody, high mobility group box 2b antibody, high mobility group box 2a antibody, HMGB2 antibody, Hmgb2 antibody, hmgb2.S antibody, hmgb2b antibody, hmgb2a antibody

Background:

Description: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:7797075, PubMed:11909973, PubMed:19522541, PubMed:18413230, PubMed:19965638, PubMed:20123072). Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (By similarity). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285). Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed:23877675). Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (By similarity). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (By similarity). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (By similarity).

Gene: HMGB2

Molecular Weight: 27 kDa

Gene ID: 3148

UniProt: P26583

Pathways: Cellular Response to Molecule of Bacterial Origin