NEK2 antibody (Internal Region)
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- Target See all NEK2 Antibodies
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NEK2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- NEK2 Antibody detects endogenous levels of total NEK2.
- Predicted Reactivity
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human NEK2, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product NEK2 Primary Antibody
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Expiry Date
- 12 months
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- Target
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
- Alternative Name
- NEK2 (NEK2 Products)
- Synonyms
- AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody
- Background
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Description: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856).
Gene: NEK2
- Molecular Weight
- 52 kDa
- Gene ID
- 4751
- UniProt
- P51955
- Pathways
- M Phase
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