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TOP3B antibody (C-Term)

TOP3B Reactivity: Human, Mouse WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6259219
  • Target See all TOP3B Antibodies
    TOP3B (Topoisomerase (DNA) III beta (TOP3B))
    Binding Specificity
    • 8
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 34
    • 20
    • 3
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 31
    • 4
    Rabbit
    Clonality
    • 32
    • 3
    Polyclonal
    Conjugate
    • 18
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TOP3B antibody is un-conjugated
    Application
    • 28
    • 17
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    TOP3B Antibody detects endogenous levels of total TOP3B.
    Predicted Reactivity
    Rabbit
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product TOP3B Primary Antibody
  • Application Notes
    WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    TOP3B (Topoisomerase (DNA) III beta (TOP3B))
    Alternative Name
    TOP3B (TOP3B Products)
    Synonyms
    TOP3B1 antibody, top3b antibody, DNA topoisomerase III beta antibody, topoisomerase (DNA) III beta antibody, DNA topoisomerase III beta L homeolog antibody, TOP3B antibody, Top3b antibody, top3b.L antibody
    Background

    Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.

    Gene: TOP3B

    Molecular Weight
    96 kDa
    Gene ID
    8940
    UniProt
    O95985
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