PHAX antibody (Internal Region)
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- Target See all PHAX Antibodies
- PHAX (phosphorylated Adaptor For RNA Export (PHAX))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PHAX antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- RNUXA Antibody detects endogenous levels of total RNUXA.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human RNUXA, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product PHAX Primary Antibody
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- Application Notes
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- PHAX (phosphorylated Adaptor For RNA Export (PHAX))
- Alternative Name
- PHAX (PHAX Products)
- Synonyms
- RNUXA antibody, 2810055C14Rik antibody, 4933427L19Rik antibody, AU018701 antibody, AU018854 antibody, D18Ertd65e antibody, Rnuxa antibody, p55 antibody, phosphorylated adaptor for RNA export antibody, PHAX antibody, Phax antibody
- Background
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Description: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
Gene: PHAX
- Molecular Weight
- 48 kDa
- Gene ID
- 51808
- UniProt
- Q9H814
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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