ADAM10 antibody (Internal Region)

Catalog No. ABIN6259766

Product Details anti-ADAM10 Antibody

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: ADAM10 Antibody detects endogenous levels of total ADAM10.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ADAM10, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10 (ADAM10 Products)

Synonyms: ADAM10 antibody, Adam10 antibody, adam10 antibody, wu:fc03d12 antibody, ad10 antibody, cd156c antibody, kuz antibody, kuzbanian antibody, madm antibody, xadam10 antibody, ADAM-10 antibody, ADAM 10 antibody, AD10 antibody, CD156c antibody, HsT18717 antibody, MADM antibody, 1700031C13Rik antibody, LOC100219653 antibody, zgc:64203 antibody, ADAM metallopeptidase domain 10 antibody, ADAM metallopeptidase domain 10a antibody, ADAM metallopeptidase domain 10 S homeolog antibody, a disintegrin and metallopeptidase domain 10 antibody, ADAM metallopeptidase domain 10b antibody, ADAM10 antibody, Adam10 antibody, adam10a antibody, adam10.S antibody, adam10b antibody

Background:

Description: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146).

Gene: ADAM10

Molecular Weight: 84 kDa

Gene ID: 102

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway