Beclin 1 antibody (Internal Region)

Catalog No. ABIN6260248

Product Details anti-Beclin 1 Antibody

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Beclin 1 Antibody detects endogenous levels of total Beclin 1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Beclin 1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Beclin 1 antibody (ABIN6260248)

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." in: OncoTargets and therapy, Vol. 11, pp. 4501-4510, (2018) (PubMed).

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: BECN1 (BECN1 Products)

Synonyms: beclin-1 antibody, atg6 antibody, vps30 antibody, beclin1 antibody, Beclin-1 antibody, becn1 antibody, zgc:73097 antibody, ATG6 antibody, VPS30 antibody, 4921513J16Rik antibody, 5430417M23Rik antibody, Atg6 antibody, beclin 1 antibody, beclin 1, autophagy related antibody, beclin 1 protein antibody, beclin-1 antibody, beclin family protein antibody, beclin 1, autophagy related L homeolog antibody, BECN1 antibody, becn1 antibody, atg6 antibody, CpipJ_CPIJ011087 antibody, PTRG_07825 antibody, SJAG_01876 antibody, Becn1 antibody, becn1.L antibody

Background:

Description: Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate, different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.

Gene: BECN1

Molecular Weight: 52 kDa

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy