SMARCA2 antibody (C-Term)

Catalog No. ABIN6260316

Product Details anti-SMARCA2 Antibody

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMARCA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Brm Antibody detects endogenous levels of total Brm.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Brm, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SMARCA2

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Alternative Name: SMARCA2 (SMARCA2 Products)

Synonyms: BAF190 antibody, BRM antibody, NCBRS antibody, SNF2 antibody, SNF2L2 antibody, SNF2LA antibody, SWI2 antibody, Sth1p antibody, hBRM antibody, hSNF2a antibody, brm antibody, 2610209L14Rik antibody, SNF2alpha antibody, Snf2l2 antibody, brahma antibody, psa-4 antibody, wu:fa56c07 antibody, wu:fi27f11 antibody, zgc:66238 antibody, Brahma antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 L homeolog antibody, SMARCA2 antibody, smarca2.L antibody, Smarca2 antibody, smarca2 antibody

Background:

Description: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Gene: SMARCA2

Molecular Weight: 181 kDa

Gene ID: 6595

UniProt: P51531