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Caspase 4 antibody (Internal Region)

CASP4 Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6260482
  • Target See all Caspase 4 (CASP4) Antibodies
    Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))
    Binding Specificity
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 58
    • 33
    • 15
    • 14
    • 1
    Human, Mouse, Rat
    Host
    • 78
    • 10
    • 1
    • 1
    • 1
    Rabbit
    Clonality
    • 77
    • 13
    Polyclonal
    Conjugate
    • 53
    • 15
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Caspase 4 antibody is un-conjugated
    Application
    • 74
    • 29
    • 27
    • 12
    • 9
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Specificity
    Caspase 4 Antibody detects endogenous levels of total Caspase 4.
    Predicted Reactivity
    Bovine,Dog
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human Caspase 4, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product CASP4 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))
    Alternative Name
    CASP4 (CASP4 Products)
    Synonyms
    ICE(rel)II antibody, ICEREL-II antibody, ICH-2 antibody, Mih1/TX antibody, TX antibody, Casp11 antibody, CASP13 antibody, CASP1 antibody, CASP4 antibody, Caspl antibody, ich-3 antibody, Casp4 antibody, caspase 4 antibody, caspase 4, apoptosis-related cysteine peptidase antibody, caspase-4 antibody, CASP4 antibody, Casp4 antibody, LOC100731032 antibody
    Background

    Description: Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion, this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).

    Gene: CASP4

    Molecular Weight
    43 kDa
    Gene ID
    837
    UniProt
    P49662
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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