CX3CL1 antibody (Internal Region)

Catalog No. ABIN6261117

Product Details anti-CX3CL1 Antibody

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: CX3CL1 Antibody detects endogenous levels of total CX3CL1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CX3CL1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: CX3CL1 (CX3CL1 Products)

Synonyms: ABCD3 antibody, CX3CL1 antibody, DKFZp459K117 antibody, Cx3c antibody, Scyd1 antibody, ABCD-3 antibody, C3Xkine antibody, CXC3 antibody, CXC3C antibody, NTN antibody, NTT antibody, SCYD1 antibody, fractalkine antibody, neurotactin antibody, AB030188 antibody, AI848747 antibody, CX3C antibody, Cxc3 antibody, D8Bwg0439e antibody, ATP binding cassette subfamily D member 3 antibody, C-X3-C motif chemokine ligand 1 antibody, chemokine (C-X3-C motif) ligand 1 antibody, ABCD3 antibody, CX3CL1 antibody, Cx3cl1 antibody, Abcd3 antibody

Background:

Description: Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350).

Gene: CX3CL1

Molecular Weight: 90-100 kDa

Gene ID: 6376

UniProt: P78423

Pathways: Synaptic Membrane