DDX21 antibody (Internal Region)

Catalog No. ABIN6261252

Product Details anti-DDX21 Antibody

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: DDX21 Antibody detects endogenous levels of total DDX21.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DDX21, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DDX21

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Alternative Name: DDX21 (DDX21 Products)

Synonyms: MGC130693 antibody, DDX21 antibody, cb960 antibody, wu:fi30c07 antibody, wu:fi34d08 antibody, GUA antibody, GURDB antibody, RH-II/GU antibody, RH-II/GuA antibody, Ddx21a antibody, AI255159 antibody, AL022742 antibody, D10Ertd645e antibody, D10Wsu42e antibody, DEAD-box helicase 21 L homeolog antibody, DExD-box helicase 21 antibody, DEAD-box helicase 21 antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody, ddx21.L antibody, DDX21 antibody, ddx21 antibody, Ddx21 antibody

Background:

Description: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77' (PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed:9461305). Involved in rRNA processing (PubMed:14559904, PubMed:18180292). May bind to specific miRNA hairpins (PubMed:28431233).

Gene: DDX21

Molecular Weight: 87 kDa

Gene ID: 9188

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interactome