E-cadherin antibody (C-Term)

Catalog No. ABIN6261408

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: E-cadherin Antibody detects endogenous levels of total E-cadherin.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human E-cadherin, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-E-cadherin antibody (ABIN6261408)

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Xu, Sun, Fang, Song, Yan, Chen, Jiang, Fei, Zhao, Leboeuf, Li, Wang, Janin, Wang, Zhao: "JAM-A overexpression is related to disease progression in diffuse large B-cell lymphoma and downregulated by lenalidomide." in: Scientific reports, Vol. 7, Issue 1, pp. 7433, (2018) (PubMed).

Guo, Yan, Yan, Guo, Zhang, Zhang, Goltzman: "Tumor-associated macrophages induce the expression of FOXQ1 to promote epithelial-mesenchymal transition and metastasis in gastric cancer cells." in: Oncology reports, Vol. 38, Issue 4, pp. 2003-2010, (2018) (PubMed).

Shi, Luo, Zhang, Wang, Zheng, Song, Wang, Zhang, Zhang, Li, Luo: "CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro." in: Thoracic cancer, Vol. 9, Issue 1, pp. 136-141, (2018) (PubMed).

Shao, Zhang, Ma, Lu, Meng, Li, Wang, Chen, Zhang, Han, Liu, Ma: "MicroRNA-139-5p affects cisplatin sensitivity in human nasopharyngeal carcinoma cells by regulating the epithelial-to-mesenchymal transition." in: Gene, Vol. 652, pp. 48-58, (2018) (PubMed).

Ran, Zhu, Deng, Zhang, Liu, Feng, Zhong, Lin, Tong, Su: "Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN." in: Journal of experimental & clinical cancer research : CR, Vol. 37, Issue 1, pp. 54, (2018) (PubMed).

Huang, Song, Tao, Shao, Zeng, Xu, Qi, Sun: "Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195610, (2018) (PubMed).

Qi, Wang, Zhao, Cai, Tang, Wang: "Cinobufacini inhibits epithelial-mesenchymal transition of human hepatocellular carcinoma cells through c-Met/ERK signaling pathway." in: Bioscience trends, Vol. 12, Issue 3, pp. 291-297, (2018) (PubMed).

Meng, Chen, Han, Qian, Wang, Zhong, Qin, Zhang, Gao, Lei, Yang, Yang, Zhang, Liu, Liu, Zhou, Sun, Yang: "Twist1 Regulates Vimentin through Cul2 Circular RNA to Promote EMT in Hepatocellular Carcinoma." in: Cancer research, Vol. 78, Issue 15, pp. 4150-4162, (2018) (PubMed).

Li, Xiao, Yang, Qin, Gao, Liu, Zhou: "Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway." in: Respiratory research, Vol. 19, Issue 1, pp. 111, (2018) (PubMed).

Wu, Chen, Xi, Wang, Sha, Luo, Zhu, Hong, Bu: "High glucose induces epithelial-mesenchymal transition and results in the migration and invasion of colorectal cancer cells." in: Experimental and therapeutic medicine, Vol. 16, Issue 1, pp. 222-230, (2018) (PubMed).

Jin, Zhang, Zhao, Xu, Shao, Huang, Shi, Yang: "Responses of Intestinal Mucosal Barrier Functions of Rats to Simulated Weightlessness." in: Frontiers in physiology, Vol. 9, pp. 729, (2018) (PubMed).

Jia, Xiao, Gongsun, Xin, Shang, Chen, Wang, Jiang: "CEP55 promotes the proliferation, migration and invasion of esophageal squamous cell carcinoma via the PI3K/Akt pathway." in: OncoTargets and therapy, Vol. 11, pp. 4221-4232, (2018) (PubMed).

Zhang, Liu, Zhang, Cui, Li, Wang, Ren, Zhang: "FOXQ1 promotes gastric cancer metastasis through upregulation of Snail." in: Oncology reports, Vol. 35, Issue 6, pp. 3607-13, (2017) (PubMed).

Bai, Wang, Han, Xie, Ji, Yin, Chen, Wang, Jiang, Qi, Jiang: "BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo." in: Molecular carcinogenesis, Vol. 56, Issue 3, pp. 1137-1149, (2017) (PubMed).

Wang, Wang, Ji, Zhu, Han: "Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy." in: Biochemical and biophysical research communications, Vol. 487, Issue 2, pp. 262-267, (2017) (PubMed).

Li, Zhao, Huang, Wang, Zhu, Cao, Xiong, Deng: "MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway." in: Gene, Vol. 641, pp. 240-247, (2017) (PubMed).

Xiang, Deng, Liu, Wan, Feng, Chen, Xiong: "MiR-1271 Inhibits Cell Proliferation, Invasion and EMT in Gastric Cancer by Targeting FOXQ1." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1382-94, (2016) (PubMed).

Li, Zhang, Sun, Sun, Shi, Liu, Liu: "MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells." in: International journal of oncology, Vol. 47, Issue 4, pp. 1379-92, (2016) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background:

Description: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Gene: CDH1

Molecular Weight: 120kDa

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation