EIF4A3 antibody (Internal Region)

Catalog No. ABIN6261488

Product Details anti-EIF4A3 Antibody

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: EIF4A3 Antibody detects endogenous levels of total eIF4A3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human eIF4A3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF4A3

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: EIF4A3 (EIF4A3 Products)

Synonyms: DDX48 antibody, NMP265 antibody, NUK34 antibody, eIF4AIII antibody, 2400003O03Rik antibody, Ddx48 antibody, eIF4A-III antibody, mKIAA0111 antibody, ddx48 antibody, nuk34 antibody, nmp265 antibody, eif4aiii antibody, EIF4A3 antibody, eif4a3 antibody, zgc:56139 antibody, XeIF-4AIII antibody, eif4a3-B antibody, eukaryotic translation initiation factor 4A3 antibody, eukaryotic initiation factor 4A-III antibody, eukaryotic translation initiation factor 4A3 S homeolog antibody, Eukaryotic initiation factor 4A-III antibody, EIF4A3 antibody, Eif4a3 antibody, eif4a3 antibody, LOC100185906 antibody, eif4a3.S antibody, if4a3 antibody

Background:

Description: ATP-dependent RNA helicase (PubMed:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:22961380, PubMed:28502770, PubMed:28076346). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly (PubMed:22203037). Involved in craniofacial development (PubMed:24360810).

Gene: EIF4A3

Molecular Weight: 47 kDa

Gene ID: 9775

UniProt: P38919