ENOS antibody (C-Term)

Catalog No. ABIN6261536

Product Details anti-ENOS Antibody

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ENOS Antibody detects endogenous levels of total eNOS.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human eNOS, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-ENOS antibody (ABIN6261536)

Hu, Cheng, Huang, Cai, Lian, Wang, Gao: "Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 42, pp. 245-257, (2018) (PubMed).

Ruan, Ren, Zhang, Zhu, Xu, Wang: "Cardioprotective Effects of QiShenYiQi Dripping Pills on Transverse Aortic Constriction-Induced Heart Failure in Mice." in: Frontiers in physiology, Vol. 9, pp. 324, (2018) (PubMed).

Zhu, Hu, Lv, Cheng, Gao: "Effects of oligomeric grape seed proanthocyanidins on L-NAME-induced hypertension in pregnant mice: Role of oxidative stress and endothelial dysfunction." in: Phytotherapy research : PTR, Vol. 32, Issue 9, pp. 1836-1847, (2018) (PubMed).

Li, Wang, Zha, Cao, Sheng, Chen: "SIRT1 inhibits TGF-β-induced endothelial-mesenchymal transition in human endothelial cells with Smad4 deacetylation." in: Journal of cellular physiology, (2018) (PubMed).

Lu, Wang, Wang, Zhang, Liu, Zhang, Geng, Shan: "Beneficial effects of renal denervation on cardiac angiogenesis in rats with prolonged pressure overload." in: Acta physiologica (Oxford, England), Vol. 220, Issue 1, pp. 47-57, (2017) (PubMed).

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3 (NOS3 Products)

Background:

Description: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Gene: NOS3

Molecular Weight: 140kDa

Gene ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling