HEY1 antibody (Internal Region)

Catalog No. ABIN6262226

Product Details anti-HEY1 Antibody

Target: HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEY1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: HEY1 Antibody detects endogenous levels of total HEY1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HEY1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HEY1

Target: HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))

Alternative Name: HEY1 (HEY1 Products)

Synonyms: BHLHb31 antibody, CHF2 antibody, HERP2 antibody, HESR1 antibody, HRT-1 antibody, OAF1 antibody, AI316788 antibody, AI414254 antibody, HRT1 antibody, Herp2 antibody, Hesr1 antibody, bHLHb31 antibody, hesr-1 antibody, id:ibd1292 antibody, zgc:110572 antibody, bc8 antibody, chf2 antibody, herp2 antibody, hesr1 antibody, hrt1 antibody, oaf1 antibody, xbc8 antibody, hes related family bHLH transcription factor with YRPW motif 1 antibody, hairy/enhancer-of-split related with YRPW motif 1 antibody, hes-related family bHLH transcription factor with YRPW motif 1 antibody, hes related family bHLH transcription factor with YRPW motif 1 S homeolog antibody, HEY1 antibody, Hey1 antibody, hey1 antibody, hey1.S antibody

Background:

Description: Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (PubMed:11095750). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3 (PubMed:15485867). Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737).

Gene: HEY1

Molecular Weight: 32-34 kDa

Gene ID: 23462

UniProt: Q9Y5J3

Pathways: Tube Formation