Ki-67 antibody (Internal Region)

Catalog No. ABIN6262794

Product Details anti-Ki-67 Antibody

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Ki67 Antibody detects endogenous levels of total Ki67.

Predicted Reactivity: Bovine,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Ki67, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Ki-67 antibody (ABIN6262794)

Liu, Huang, Jin, Zhou, Chen, Li, Chen, Li, Yao, Li, Lan, Ye, Wang: "MicroRNA-370 inhibits the growth and metastasis of lung cancer by down-regulating epidermal growth factor receptor expression." in: Oncotarget, Vol. 8, Issue 50, pp. 88139-88151, (2018) (PubMed).

Yang, Ren, Zhang, Li, Cheng, Xia: "Oridonin inhibits oral cancer growth and PI3K/Akt signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 100, pp. 226-232, (2018) (PubMed).

Gong, Fan, Wang, Han, Lv, Xu, Chen, He: "Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis." in: World journal of gastroenterology, Vol. 24, Issue 16, pp. 1766-1778, (2018) (PubMed).

Zhu, Cai, Guo, Chen, Yi, Zhao, Cai, Wang: "Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression." in: Tumour biology, Vol. 37, Issue 10, pp. 14009-14023, (2017) (PubMed).

Sun, Huang, Li, Yang, Xi, Wang, Zhang, Fu, Li: "Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development." in: Oncotarget, Vol. 7, Issue 7, pp. 8341-59, (2016) (PubMed).

Chen, Liang, Zhu, Fu, Chen, Mo, Zhou, Fu, Bi, He, Yang, Wu, Jiang, Zhong: "CC chemokine ligand 18 correlates with malignant progression of prostate cancer." in: BioMed research international, Vol. 2014, pp. 230183, (2014) (PubMed).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: MKI67 (MKI67 Products)

Synonyms: MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody

Background:

Description: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Gene: MKI67

Molecular Weight: 358kDa

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process