LATS2 antibody (Internal Region)

Catalog No. ABIN6262923

Product Details anti-LATS2 Antibody

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Binding Specificity: Internal Region

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LATS2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: LATS2 Antibody detects endogenous levels of total LATS2.

Predicted Reactivity: Pig,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LATS2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LATS2

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Alternative Name: LATS2 (LATS2 Products)

Synonyms: 4932411G09Rik antibody, AV277261 antibody, AW228608 antibody, KPM antibody, lats2 antibody, large tumor suppressor kinase 2 antibody, large tumor suppressor 2 antibody, LATS2 antibody, Lats2 antibody, lats2 antibody

Background:

Description: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

Gene: LATS2

Molecular Weight: 150 kDa

Gene ID: 26524

UniProt: Q9NRM7