LCK antibody (Lymphocyte-Specific Protein tyrosine Kinase)

Details for Product anti-LCK Antibody No. ABIN6262930
Antigen
  • zgc:136695
  • LCK
  • Hck-3
  • Lsk
  • Lskt
  • p56
  • p56Lck
  • LSK
  • YT16
  • p56lck
  • pp58lck
  • P56LCK
  • tkl
  • Lck1
  • Lcktkr
  • LCK proto-oncogene, Src family tyrosine kinase
  • lymphocyte protein tyrosine kinase
  • lck
  • LCK
  • Lck
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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236
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10
8
8
4
4
4
3
2
1
Host
Rabbit
331
67
9
Clonality
Polyclonal
Conjugate
This LCK antibody is un-conjugated
14
10
9
6
6
6
6
6
6
6
6
5
2
2
2
2
2
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
345
173
87
57
49
35
35
26
21
12
4
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Options
Immunogen A synthesized peptide derived from human LCK
Isotype IgG
Specificity LCK Antibody detects endogenous levels of total LCK
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Plasmids, Primers & others Plasmids, Primers & others LCK products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name LCK (LCK Antibody Abstract)
Background

Description: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501).

Gene: LCK

Molecular Weight 56kDa
Gene ID 3932
UniProt P06239
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) antibody (ABIN6262930) Western blot analysis of Jurkat whole cell lysates, using LCK Antibody. The lane on t...
Image no. 2 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) antibody (ABIN6262930) ABIN6277159 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeab...
Image no. 3 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) antibody (ABIN6262930) ABIN6277159 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyd...
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