NUDT21 antibody (Internal Region)

Catalog No. ABIN6263764

Product Details anti-NUDT21 Antibody

Target: NUDT21 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 21 (NUDT21))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUDT21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: NUDT21 Antibody detects endogenous levels of total NUDT21.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NUDT21, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NUDT21

Target: NUDT21 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 21 (NUDT21))

Alternative Name: NUDT21 (NUDT21 Products)

Synonyms: CFIM25 antibody, CPSF5 antibody, 25kDa antibody, 3110048P04Rik antibody, 5730530J16Rik antibody, AU014860 antibody, AW549947 antibody, Cpsf5 antibody, zgc:63966 antibody, cpsf5 antibody, An16g01870 antibody, AO090003001316 antibody, Afu5g02030 antibody, T9L24.30 antibody, T9L24_30 antibody, atnudt21 antibody, nudix hydrolase homolog 21 antibody, nudix hydrolase 21 antibody, nudix (nucleoside diphosphate linked moiety X)-type motif 21 antibody, nudix hydrolase 21 L homeolog antibody, cleavage and polyadenylation specificity factor subunit 5 antibody, cleavage and polyadenylation specific factor 5 antibody, autocrine motility factor receptor antibody, nudix hydrolase homolog 21 antibody, NUDT21 antibody, Nudt21 antibody, nudt21 antibody, nudt21.L antibody, ANI_1_1518144 antibody, AOR_1_2312154 antibody, PTRG_11035 antibody, PAAG_04662 antibody, MCYG_02022 antibody, VDBG_06706 antibody, MGYG_04057 antibody, cpsf5 antibody, PGTG_05353 antibody, Tsp_03672 antibody, LOC732888 antibody, AFUA_5G02030 antibody, NFIA_040090 antibody, ACLA_003240 antibody, CC1G_07993 antibody, PMAA_039710 antibody, AFLA_025180 antibody, TSTA_079060 antibody, BDBG_01565 antibody, TERG_04393 antibody

Background:

Description: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:15937220, PubMed:17024186, PubMed:17098938, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:17024186). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:17098938, PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:17098938, PubMed:20695905, PubMed:29276085). NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7 (PubMed:29276085). Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing (PubMed:8626397, PubMed:14690600, PubMed:15169763, PubMed:17024186, PubMed:22813749, PubMed:20479262). The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (PubMed:20479262, PubMed:21295486). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (PubMed:18445629).

Gene: NUDT21

Molecular Weight: 26 kDa

Gene ID: 11051

UniProt: O43809