Occludin antibody

Catalog No. ABIN6263796

Product Details anti-Occludin Antibody

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Occludin Antibody detects endogenous levels of total Occludin

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Occludin

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000 IHC 1:200 IF 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-Occludin antibody (ABIN6263796)

Ai, Zhang, Gao, Qin, Zhong, Gu, Li, Qiao, Tian, Cui, Yang, Bi, Xiao, Chen, Liu, Zhou, Sun, Yang: "Sesquiterpene binding Gly-Leu-Ser/Lys-"co-adaptation pocket" to inhibit lung cancer cell epithelial-mesenchymal transition." in: Oncotarget, Vol. 8, Issue 41, pp. 70192-70203, (2018) (PubMed).

Wang, Lu, Xie, Xiong, Huang, Wei, Jiang, Peng: "Blend of organic acids and medium chain fatty acids prevents the inflammatory response and intestinal barrier dysfunction in mice challenged with enterohemorrhagic Escherichia coli O157:H7." in: International immunopharmacology, Vol. 58, pp. 64-71, (2018) (PubMed).

Zou, Xiang, Wang, Peng, Wei: "Oregano Essential Oil Improves Intestinal Morphology and Expression of Tight Junction Proteins Associated with Modulation of Selected Intestinal Bacteria and Immune Status in a Pig Model." in: BioMed research international, Vol. 2016, pp. 5436738, (2017) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin (OCLN Products)

Synonyms: AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody

Background:

Description: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

Gene: OCLN

Molecular Weight: 62 kDa

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C