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PHF1 antibody (Internal Region)

PHF1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6264170
  • Target See all PHF1 Antibodies
    PHF1 (PHD Finger Protein 1 (PHF1))
    Binding Specificity
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 33
    • 16
    • 16
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 30
    • 6
    Rabbit
    Clonality
    • 31
    • 5
    Polyclonal
    Conjugate
    • 26
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PHF1 antibody is un-conjugated
    Application
    • 26
    • 14
    • 9
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    PHF1 Antibody detects endogenous levels of total PHF1.
    Predicted Reactivity
    Pig,Horse,Sheep,Rabbit
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human PHF1, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product PHF1 Primary Antibody
  • Application Notes
    WB: 1:500-1:3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    PHF1 (PHD Finger Protein 1 (PHF1))
    Alternative Name
    PHF1 (PHF1 Products)
    Synonyms
    pcl1 antibody, xpcl1 antibody, MTF2L2 antibody, PCL1 antibody, PHF2 antibody, TDRD19C antibody, hPHF1 antibody, AW557215 antibody, D17Ertd455e antibody, Tctex-3 antibody, Tctex3 antibody, mPcl1 antibody, PHD finger protein 1 L homeolog antibody, PHD finger protein 1 antibody, phf1.L antibody, PHF1 antibody, Phf1 antibody
    Background

    Description: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.

    Gene: PHF1

    Molecular Weight
    62kDa
    Gene ID
    5252
    UniProt
    O43189
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