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PIM1 antibody (Internal Region)

PIM1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6264220
  • Target See all PIM1 Antibodies
    PIM1 (Pim-1 Oncogene (PIM1))
    Binding Specificity
    • 19
    • 16
    • 15
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 103
    • 47
    • 46
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 104
    • 9
    Rabbit
    Clonality
    • 93
    • 20
    Polyclonal
    Conjugate
    • 46
    • 16
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This PIM1 antibody is un-conjugated
    Application
    • 95
    • 51
    • 28
    • 27
    • 26
    • 18
    • 14
    • 8
    • 6
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Pim-1 Antibody detects endogenous levels of total Pim-1.
    Predicted Reactivity
    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human Pim-1, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product PIM1 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    PIM1 (Pim-1 Oncogene (PIM1))
    Alternative Name
    PIM1 (PIM1 Products)
    Synonyms
    PIM antibody, Pim-1 antibody, PIM1 antibody, pim antibody, pim-1 antibody, pim3 antibody, Pim-1 proto-oncogene, serine/threonine kinase antibody, proviral integration site 1 antibody, Pim-1 proto-oncogene, serine/threonine kinase L homeolog antibody, PIM1 antibody, Pim1 antibody, pim1 antibody, pim1.L antibody
    Background

    Description: Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

    Gene: PIM1

    Molecular Weight
    50kDa
    Gene ID
    5292
    UniProt
    P11309
    Pathways
    Glycosaminoglycan Metabolic Process
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