POLR2E antibody (Internal Region)
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- Target See all POLR2E Antibodies
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR2E antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- POLR2E Antibody detects endogenous levels of total POLR2E.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human POLR2E, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product POLR2E Primary Antibody
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- Alternative Name
- POLR2E (POLR2E Products)
- Synonyms
- MGC68604 antibody, polr2e antibody, wu:fk97g10 antibody, zgc:101098 antibody, 2410021N14Rik antibody, 25kDa antibody, AW208866 antibody, RPB5 antibody, XAP4 antibody, RPABC1 antibody, hRPB25 antibody, hsRPB5 antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog antibody, RNA polymerase II subunit E antibody, polymerase (RNA) II (DNA directed) polypeptide E, b antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, polymerase (RNA) II (DNA directed) polypeptide E antibody, DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, polr2e.S antibody, POLR2E antibody, polr2eb antibody, polr2e antibody, Polr2e antibody, rpb-5 antibody
- Background
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Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).
Gene: POLR2E
- Molecular Weight
- 24kDa
- Gene ID
- 5434
- UniProt
- P19388
- Pathways
- Regulatory RNA Pathways
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