POLR2I antibody (Internal Region)

Catalog No. ABIN6264346

Product Details anti-POLR2I Antibody

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: POLR2I Antibody detects endogenous levels of total POLR2I.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human POLR2I, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR2I

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Alternative Name: POLR2I (POLR2I Products)

Synonyms: RPB9 antibody, hRPB14.5 antibody, GB18029 antibody, RPB14.5 antibody, 2810002B19Rik antibody, zgc:103515 antibody, RNA polymerase II subunit I antibody, DNA-directed RNA polymerase II subunit RPB9 antibody, DNA-directed RNA polymerase II 14.5 kDa polypeptide antibody, polymerase (RNA) II (DNA directed) polypeptide I antibody, polymerase (RNA) II subunit I L homeolog antibody, POLR2I antibody, LOC412547 antibody, LOC100282092 antibody, Polr2i antibody, polr2i antibody, polr2i.L antibody

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity).

Gene: POLR2I

Molecular Weight: 15kDa

Gene ID: 5438

UniProt: P36954

Pathways: Regulatory RNA Pathways