POLR2G antibody (Internal Region)

Catalog No. ABIN6264819

Product Details anti-POLR2G Antibody

Target: POLR2G (Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2G antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: RPB7 Antibody detects endogenous levels of total RPB7.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPB7, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR2G

Target: POLR2G (Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G))

Alternative Name: POLR2G (POLR2G Products)

Synonyms: RPB19 antibody, RPB7 antibody, hRPB19 antibody, hsRPB7 antibody, Rpb7 antibody, POLR2G antibody, 2410046K11Rik antibody, A230108L04Rik antibody, C76415 antibody, RBP7 antibody, Rpo2-7l antibody, RNA polymerase II subunit G antibody, polymerase (RNA) II (DNA directed) polypeptide G antibody, polymerase (RNA) II (DNA directed) polypeptide G L homeolog antibody, POLR2G antibody, Polr2g antibody, polr2g antibody, polr2g.L antibody

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.

Gene: POLR2G

Molecular Weight: 19 kDa

Gene ID: 5436

UniProt: P62487

Pathways: Regulatory RNA Pathways