S100A12 antibody

Catalog No. ABIN6264899

Product Details anti-S100A12 Antibody

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A12 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Specificity: S100A12 Antibody detects endogenous levels of total S100A12

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human S100A12

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Alternative Name: S100A12 (S100A12 Products)

Synonyms: CAAF1 antibody, CAGC antibody, CGRP antibody, ENRAGE antibody, MRP-6 antibody, MRP6 antibody, p6 antibody, CO-AG antibody, EN-RAGE antibody, S100 calcium binding protein A12 antibody, protein S100-A12 antibody, S100A12 antibody, LOC101103771 antibody

Background:

Description: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases, MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

Gene: S100A12

Molecular Weight: 10kDa

Gene ID: 6283

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteins