SETD2 antibody (SET Domain Containing 2)

Details for Product anti-SETD2 Antibody No. ABIN6265018
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
75
18
15
3
2
2
Host
Rabbit
62
9
7
Clonality
Polyclonal
Conjugate
This SETD2 antibody is un-conjugated
6
4
4
4
4
4
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
41
38
37
16
6
4
3
2
1
1
Options
Immunogen A synthesized peptide derived from human SETD2
Isotype IgG
Specificity SETD2 Antibody detects endogenous levels of total SETD2
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Antigen
Alternative Name SETD2 (SETD2 Antibody Abstract)
Background

Description: Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:16118227, PubMed:19141475, PubMed:21526191, PubMed:21792193, PubMed:23043551, PubMed:27474439). Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation (By similarity). Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A (PubMed:23325844). Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction (PubMed:23622243). Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR) (PubMed:24843002). Acts as a tumor suppressor (PubMed:24509477). H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A (PubMed:27317772). H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase (PubMed:21792193). Required during angiogenesis (By similarity). Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3 (By similarity). In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1 (PubMed:27518565, PubMed:28753426). Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3), alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling (PubMed:27518565). Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription (PubMed:28753426).

Gene: SETD2

Molecular Weight 28kDa
Gene ID 29072
UniProt Q9BYW2
Pathways Tube Formation
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-SET Domain Containing 2 (SETD2) antibody (ABIN6265018) Western blot analysis of Hela cell lysate, using SETD2 Antibody. The lane on the left...
Image no. 2 for anti-SET Domain Containing 2 (SETD2) antibody (ABIN6265018) ABIN6277490 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was for...