SPAG5 antibody (Internal Region)

Catalog No. ABIN6265251

Product Details anti-SPAG5 Antibody

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: SPAG5 Antibody detects endogenous levels of total SPAG5.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SPAG5, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SPAG5

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Alternative Name: SPAG5 (SPAG5 Products)

Synonyms: SPAG5 antibody, DEEPEST antibody, MAP126 antibody, hMAP126 antibody, AI874642 antibody, D11Bhm180e antibody, Deepest antibody, Mastrin antibody, S17 antibody, sperm associated antigen 5 antibody, sperm-associated antigen 5 antibody, SPAG5 antibody, LOC739263 antibody, Spag5 antibody

Background:

Description: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).

Gene: SPAG5

Molecular Weight: 134 kDa

Gene ID: 10615

UniProt: Q96R06

Pathways: M Phase