TJP3 antibody (C-Term)

Catalog No. ABIN6265590

Product Details anti-TJP3 Antibody

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: TJP3 Antibody detects endogenous levels of total TJP3.

Predicted Reactivity: Pig,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TJP3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Alternative Name: TJP3 (TJP3 Products)

Synonyms: cb766 antibody, wu:fj93c08 antibody, zo-3 antibody, zo3 antibody, ZO-3 antibody, ZO3 antibody, tight junction protein 3 antibody, tight junction protein 3 S homeolog antibody, tjp3 antibody, tjp3.S antibody, TJP3 antibody, Tjp3 antibody

Background:

Description: TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed:16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With TJP1 and TJP2, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).

Gene: TJP3

Molecular Weight: 101 kDa

Gene ID: 27134

UniProt: O95049