UBE2D1 antibody (C-Term)

Catalog No. ABIN6265844

Product Details anti-UBE2D1 Antibody

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: UBE2D1 Antibody detects endogenous levels of total UBE2D1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human UBE2D1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for UBE2D1

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Alternative Name: UBE2D1 (UBE2D1 Products)

Synonyms: E2(17)KB1 antibody, SFT antibody, UBC4/5 antibody, UBCH5 antibody, UBCH5A antibody, ube2d1 antibody, wu:fc16h06 antibody, zgc:73096 antibody, e2(17)kb1 antibody, sft antibody, ubc4/5 antibody, ubch5 antibody, ubch5a antibody, ubiquitin conjugating enzyme E2 D1 antibody, ubiquitin-conjugating enzyme E2D 1 antibody, ubiquitin-conjugating enzyme E2D 1b antibody, ubiquitin conjugating enzyme E2 D1 S homeolog antibody, UBE2D1 antibody, Ube2d1 antibody, ube2d1b antibody, ube2d1.S antibody, ube2d1 antibody

Background:

Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:20061386). Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1 (PubMed:18042044, PubMed:18359941). Ubiquitinates STUB1-associated HSP90AB1 in vitro (PubMed:18042044). Lacks inherent specificity for any particular lysine residue of ubiquitin (PubMed:18042044). Essential for viral activation of IRF3 (PubMed:19854139). Mediates polyubiquitination of CYP3A4 (PubMed:19103148).

Gene: UBE2D1

Molecular Weight: 17kDa

Gene ID: 7321

UniProt: P51668

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades