VEGFA antibody (Internal Region)

Catalog No. ABIN6265963

Product Details anti-VEGFA Antibody

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: VEGFA Antibody detects endogenous levels of total VEGFA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human VEGFA, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-VEGFA antibody (ABIN6265963)

Liu, Chen, Jiang, Guo, Xie, Kang, Cheng: "Berberine inhibits the LPS-induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 6125-6130, (2018) (PubMed).

Li, Ma, Zhang: "CoCl2 increases the expression of hypoxic markers HIF-1α, VEGF and CXCR4 in breast cancer MCF-7 cells." in: Oncology letters, Vol. 15, Issue 1, pp. 1119-1124, (2018) (PubMed).

Yin, Wen, Lai, Li, Wang: "Lenalidomide improvement of cisplatin antitumor efficacy on triple-negative breast cancer cells in vitro." in: Oncology letters, Vol. 15, Issue 5, pp. 6469-6474, (2018) (PubMed).

Chen, Chen, Xu, Zhong, Zhang, Yang, Huang: "Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation." in: Biology of reproduction, (2018) (PubMed).

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." in: OncoTargets and therapy, Vol. 11, pp. 4501-4510, (2018) (PubMed).

Ai, Liu, Lu, Liang, Sun, Chen, Sun, Li, Liu, Zhang, Liu, Xiao, Jing, Sun, Zhou, Yang: "Phenytoin silver: a new nanocompound for promoting dermal wound healing via comprehensive pharmacological action." in: Theranostics, Vol. 7, Issue 2, pp. 425-435, (2017) (PubMed).

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA (VEGFA Products)

Synonyms: MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody

Background:

Description: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

Gene: VEGFA

Molecular Weight: 28 kDa

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling