VPS54 antibody (Internal Region)

Catalog No. ABIN6265999

Product Details anti-VPS54 Antibody

Target: VPS54 (Vacuolar Protein Sorting 54 Homolog (VPS54))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS54 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: VPS54 Antibody detects endogenous levels of total VPS54.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human VPS54, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for VPS54

Target: VPS54 (Vacuolar Protein Sorting 54 Homolog (VPS54))

Alternative Name: VPS54 (VPS54 Products)

Synonyms: 5330404P15Rik antibody, Hcc8 antibody, Vps54l antibody, mSLP8 antibody, wr antibody, HCC8 antibody, SLP-8p antibody, VPS54L antibody, WR antibody, hVps54L antibody, Vsp54 antibody, Vacuolar protein sorting-associated protein 54 antibody, VPS54 GARP complex subunit antibody, VPS54, GARP complex subunit antibody, vps-54 antibody, Vps54 antibody, VPS54 antibody

Background:

Description: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).

Gene: VPS54

Molecular Weight: 111 kDa

Gene ID: 51542

UniProt: Q9P1Q0